Extracellular Biocatalytic Activity of Thermophilic Lipase And Protease of Solibacillus Silvestris NND-3
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Abstract
In this present study, thermophilic Solibacillus silvestris NND-3 was isolated from hot-water spring of Atri region of Odisha state, present in India and evaluated for extracellular biocatalytic activity of thermophilic lipase and protease by using culture dependent approaches. Protease activity was observed optimum at the stationary phase, 36-hours (remain stable for 24-36 hours at 560C) of NND-3 isolate. Effect of organic solvents and various inhibitors and commercial detergents on residual protease activities (mean %) of NND-3 isolate were observed maximum as compared to residual lipase activity (mean %). 16S rRNA sequence of the thermophilic bacterial isolate was deposited in NCBI database and obtained GenBank accession number as JX680812. This thermophilic bacterial strain was emerged as one among few strains having high protease productivity in Firmicutes class with novel bioprospecting potential which can be used in detergent based industries.
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